Validation Of An Lc-ms/ms Method For The Determination Of Quetiapine, Norquetiapine And Quetiapine Sulphoxide In Human Whole Blood Using The Dried Blood Spot Technique

Neil Doleman - Principal Investigator
Robin Lane - Senior Analyst

Background

The Dried Blood Spot (DBS) technique has been evaluated at BASi® UK for the determination of quetiapine, norquetiapine and quetiapine sulphoxide in human whole blood. DBS offers a range of benefits over traditional sampling techniques including:

  • Refinement - elimination/reduction of rodent warming
  • Reduction - reduced volumes enables serial sampling from one rodent reducing animal numbers and eases sampling in paediatric studies
  • Reduced costs - animal numbers, test substance, simplified procedures (collection and laboratory), shipping and storage

Objective

To validate an analytical method in human whole blood achieving an LLOQ of 0.5 ng/mL for all analytes at BASi® UK prior to transferring the method to our US sites and performing a cross-validation exercise.