ADME Studies

BASi® is proud to offer services using our own BASi Culex® Automated In Vivo Sampling System. The BASi Culex ® allows simultaneous programmed sampling of blood and other body fluids. This integrative approach reduces animal usage while providing stronger data.  BASi® offers a full range of ADME studies and we’ll work closely with you to design the study that exactly suits your needs.

Study designs can be highly variable with multiple dosing days and dosing routes available.  Samples can be collected at any time of the day or night.  Blood collection can be combined with microdialysis, urine and feces collection, and behavioral collection.

Sample analysis is available in our GLP laboratories for test compounds, metabolites, neurotransmitters, hormones and more. In some cases, related compounds can be co-processed while maintaining sample integrity. Multi-parameter assays are also possible for some neurotransmitters.

Study Examples:

• Pharmacokinetics
• IV/Oral Bioavailability
• Tissue Distribution - Simultanteous PK and Microdialysis
• First Pass Metabolism

Pharmacokinetics

• This technique is available in rats and mice.
• Sample sizes range from 5uL up to 1mL per sample with samples as close as every 3 minutes.
• Blood collection options include carotid artery, jugular vein, femoral vein, femoral artery and portal vein (in mice, carotid and jugular collection only).

  

 

Bioavailability

• This technique is available in rats and mice, with two complete curves derived from each subject.
• Sample sizes range from 5uL up to 1mL per sample with samples as close as every 3 minutes.
• Dosing is performed with an automated infusion system to minimize human interaction and stress.

Simultaneous PK and Microdialysis

• This technique is available in rats and mice.
• Blood sampling catheters are combined with microdialysis or ultrafiltration probes in target tissues to compare systemic exposure with tissue distribution.
• Microdialysis options are highly diverse and include brain, liver, fat, subcutaneous tissue, peritoneal cavity, and in some cases, lung.
• An alternative technique is available with whole organ collection.
  Blood collection options include carotid artery, jugular vein, femoral vein, femoral artery and portal vein (in mice, carotid and jugular collection only).

   

 

First Pass Metabolism

• This technique is available in rats only.
• Blood collection options include the portal vein and a peripheral vessel of your choice.
• Samples are collected simultaneously from both vessels to measure how much drug is absorbed from the gut and also how much passes through the liver to the systemic circulation.
• The typical dosing method for this study is via intra-gastric catheter and is automated. Oral gauvage dosing is also possible.