New Approach for Managing Blood Waste
Disposing of Waste Blood
The BASi Culex ® Automated Blood Sampler uses sterile catheters connected to sterile disposable tubing on the instrument to (a) withdraw blood from the animal, (b) divert an aliquot to the collection vials, (c) and return any blood remaining in the catheter to the animal with a following flush of sterile saline to replace the fluids withdrawn. Using the standard BASi Culex ® software* there is 50µL of blood remaining in the fraction collector tubing at the end of each sampling sequence. This is considered “waste” and is not returned to the animal. Instead, it is flushed with saline into a waste line which starts inside the fraction collector and terminates at a waste collection bottle underneath the cart.
This approach to waste management requires that the user routinely clean out the fraction collector line after each experiment. The procedure involves removal of the sample carousel, a squeeze bottle with a long nozzle, and a cotton swab. The cleaning solution is 10% bleach (10 mL Chlorox bleach** in 90 mL water). The goal is to flush any blood residue down the collection line and prevent the growth of microbes in the waste line.
Problems Associated with Cleaning The Waste Line
In our experience, this cleanout procedure is sometimes overlooked, which can create a problem with blockage due to vigorous microbial growth in the waste line. In other cases, the cleanup is actually too vigorous and some users have sprayed or spilled cleaning solution into other parts of the fraction collector, compromising the function of temperature sensors and electronics. In some cases, users have used other cleaning solutions containing organic solvents (e.g. alchohols) which have dissolved the adhesives used to support the waste line and created leakage inside the fraction collector.
Using Radioisotopes or Infected Animal Models
Some users have developed specific needs which are not well served by the aforementioned cleaning method. The present cleaning method could expose the user to blood from the animal and this is not desirable when:
A New Approach
We have developed another approach to deal with blood waste. This is currently considered “experimental”. We are seeking feedback from BASi Culex ® users to determine how successfully this approach can be transferred to other laboratories. If the feedback is positive, we will incorporate this new approach by modifying the contents of the standard BASi Culex ® tubing set. This new approach accomplishes the following goals:
This new approach also requires caution by users. The BASi Culex ® tubing set was intended to be used for one animal, and is warranted for that purpose. We have found some laboratories reusing the same tubing sets for several animals. Repeated use of the new blood waste technique beyond an estimated maximum of 30 samples will probably lead to failure of the waste collection technique. Thirty samples should be more than enough to accommodate the use of one rodent, even with duplicate sets of blood samples as in a bioavailability study. Users who continue to re-use the same tubing set will encounter serious problems with leakage of waste material into the fraction collector.
CX-5030 BASi Culex ® Waste Containment Kit
Each waste containment kit includes the following 4 components:
The 4th component required for this kit is a waste bag. The best choice is to recycle an old saline bag from a previous BASi Culex ® experiment. Please do not use glass serum bottles!
Instructions for Use
* New software employing the “no-waste” method -- which leaves no blood in this line ---- was developed for mice and is now being developed for rats (Win/98 and Win X/P version) for a January, 2004. New users (and old users getting software upgrades) will notice a prototype version on their BASi Culex ® software disk
** Bleach is the common name for sodium hypochlorite -- NaOCl