Data Collection with Non-BASi Software

Some users would prefer to use their own data-collection systems, either because they are in a regulated environment or because they are using the epsilon detector in an existing LC system that has its own data collection and processing. This can be accomplished by using the analog outputs of the epsilon to send the signal to the other system, and configuring the epsilon to accept an external rezero from the other system. You will have to use BASi Control software to set the Epsilon's parameters, turn on the cell, etc.

 

 

• CONNECTING THE ANALOG OUTPUTS
• EXTERNAL REZERO
• CONTROLLING THE EPSILON
• PUTTING IT ALL TOGETHER

 

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CONNECTING THE ANALOG OUTPUTS

The epsilon provides a 1-Volt full scale analog output (pictured here) for connection to an external data system. Please be sure to:

 

1. Connect Wn on the epsilon to (+) or High on the data system.
2. Connect Gnd on the epsilon to (-) or Low on the data system.
3. Set the data system for an input of 1 Volt (or an attenuation of 10).
4. NOT use any additional ground connections at the data system.

 

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EXTERNAL REZERO

It is important to rezero the epsilon during unattended operation, to prevent the baseline from drifting offscale. This is most conveniently done just before each injection. Most autosamplers and data systems provide a means to send a suitable trigger to peripheral equipment. The epsilon detector requires a momentary switch closure, or a TTL-low, to its TTL-INPUTS connector to accomplish this.

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CONTROLLING THE EPSILON

The epsilon detector must be controlled by BASi Control software. You will be using MANUAL OPERATION in the EC SCHEDULE to WARMUP, and then EXEC, the detector with the parameters found in the first line of the EC Schedule.

Be sure to read the entire section on OPERATION WITH CHROMGRAPH CONTROL to fully understand BASi Control software. You will generally not be using the procedures discussed in DATA COLLECTION WITH CHROMGRAPH CONTROL, nor those in DATA ANALYSIS WITH CHROMGRAPH REPORT.

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PUTTING IT ALL TOGETHER

The sequence for making this work is as follows:

 

1. Assemble and connect the FLOWCELL.

    

2. Start the pump.

    

3. WARMUP the detector and allow it to equilibrate for as many hours as necessary.

    

4. EXEC and ZERO the detector.

    

5. From this point on the autosampler and data system take control: the autosampler triggers a rezero, triggers the data system, and injects.

 

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