Evaluation Of The Dried Blood Spot Technique For The Quantification Of Diltiazem In Human Blood

Neil Doleman
BASi®, Suite 2 Building 500, Abbey Park, Stareton, Kenilworth, Warwickshire, UK

Background

There is renewed interest in the development of the Dried Blood Spot technique (DBS) for determining drug levels in blood samples. The benefits of this sampling technique for pre-clinical and clinical bioanalytical assays have been recognised by BASi®.

• Refinement - elimination/reduction of rodent warming
  
• Reduction - reduced volumes enables serial sampling from one rodent reducing animal numbers and eases sampling in paediatric studies
  
• Reduced costs - animal numbers, test substance, simplified procedures (collection and laboratory), shipping and storage

Objective

To evaluate the use of DBS to determine diltiazem in human blood.