Application Guide 


A Complete Solution Kit for Research & Analysis in Multiple Areas of Bio-Sensing Such as Detection of Pesticides, Heavy Metals, Drugs, Neurotoxic Gases, Natural Toxins and Forensic Science




The AChE Enzyme Modified Bio-Sensor

The Bio-Sensor Connector Options for PalmSens Potentiostats

The Dual Compatible Electrochemical Glass Cell for SPE as well as regular Three – Electrode Based Sensor Measurements

The Thermostat Apparatus for Cooling/heating of temperature sensitive sensor electrolytes



The AChE Enzyme Modified Bio-Sensor

Amperometric Acetylcholinesterase three-electrode sensor with patented structure made by thick film technology

Description: Acetylcholinesterase from electric eel type VI-S is immobilized on the active surface of a working electrode of electrochemical sensor SP-1211-AC (i.e. Platinum working electrode, silver reference electrode). The diameter of the immobilized bioactive membrane is 2 mm and the mean applied activity is 1 unit/mm2. This screen printed amperometric sensor has three electrodes and is formed on a corundum base. It is 2.54 cm long, 0.73 cm wide, 0.63 mm thick, and weights 0.5 grams. The diameter of the platinum working electrode area is 1.00 mm. There is a silver electrical contact at the end of each sensor and this conductive path is covered by a dielectric protection layer. A biochemically active enzyme is immobilized on the surface of the working electrode area to create the biosensor. They can be ordered in multiples of 20 sensors. The PALM-SPEHOLDER-2MM is an appropriate adapter for these sensors.

Application Areas: Measurement of AChE inhibitor concentration – organophosphorous and carbamate pesticide (paraoxon, malaoxon) analysis, toxic and neurotoxic gases (sarin, soman, tabun, VX) research, heavy metals (copper, aluminum) detection, drugs (donepezil, rivastigmin, huperzine) analysis and secondary metabolites and natural toxins (caffeine, aflatoxin) research.




[1] Z. Grosmanová, J. Krejčí, J. Týnek, P. Cuhra, S. Baršová: Comparison of biosensoric and chromatographic methods for the detection of pesticides. International Journal of Environmental Analytical Chemistry 85(2005) 885-893

[2] J. Haze, M. Skocdopole, J. Krejci, R. Vrba, L. Fujcik, D. Krejcova, Z. Grosmanova: Artificial synapsis - The detector of pesticide toxicity. XVII IMEKO Word Congress Metrology (2003)



The Bio-Sensor Connector Options for PalmSens Potentiostats

PALM-SPEHOLDER-2MM: PalmSens Screen Printed Electrode Connector for Drop Based Measurements

This economical connector is a simple device with 3 banana receptacles and a connection for the screen-printed electrodes. It is compatible with any screen-printed electrode that has 2.54 mm spacing between the contact pads.



Sensor Cable for BVT-AC1 SPE for Applications involving EC Cells

This connector fits perfectly into the cells produced by BVT and this way offers a convenient tool to fix your electrodes. The connector enables the use of the BASi provided screen printed electrodes in a classic electrochemical arrangement with a stirred vessel.




The Dual Compatible Electrochemical Glass Cell for SPE as well as regular Three – Electrode Based Sensor Measurements

BVT-TC7: Electrochemical Glass Cell with Manual Stirrer and Multiple Options

The TC7 is a low cost Glass Cell that is ideal for students and basic lab measurements. The TC7 comes with a stand, specially designed for it, and the stand provides more stability and ease of use. It contains:

​   - A central opening (NJ 12/14 for stirrer)
​   - Four side openings (NJ 10/13 for connectors or classic electrodes).
​   - The three dimensional stand provides more stability.

These openings can be used to mount working, reference and counter electrodes as well as other accessories. This simple glass cell is used for electrochemical measurements. The minimum volume of a sample solution inside the cell is 5 mL.



The Thermostat Apparatus for Cooling/heating of temperature sensitive sensor electrolytes

BVT-MT1: Minithermostat for Electrochemical Measurements

Technical Parameters

   - Supply: 12 V
   - Min temperature: -9.9°C
   - Max temperature: 59.9 °C

Device Usage

​   - Cooling/heating of solution of temperature sensitive chemicals during experiments
​   - Maintaining of chemicals at exact temperature
​   - The electrochemical measurement with thermodiffusion
​   - Heated electrodes technology
​   - The option with Eppendorf microvials 0.2 ml, 0.5 ml, 1.5 ml is possible

Minithermostat device is designed for tempering an electrolytic bath for electrochemical measurements. The temperature is stabilized and the Peltier thermal element, which allows cool or warm the measured electrolytic samples.