Laboratory Training Manual of
Microdialysis and In Vivo UF Sampling
Chapter 1: Overview of In Vivo Bioanalytical Sampling Techniques
 1-1. The Ideal Tool
1-2. Open Cannula Techniques
1-3. Microdialysis
1-4. Ultrafiltration
Chapter 2: Preparation for Microdialysis
2-1. Selecting a Probe
2-2. Instrumentation Requirements
2-3. Perfusion Fluids
2-4. Preparing and Plumbing Microdialysis Probes
2-5. In Vitro Recovery
2-6. An In Vitro Recovery Experiment
Chapter 3: Setting Up a Microdialysis System: An Overview
3-1. Start-Up
3-2. Surgery
3-3. Perfusion System
3-4. Animal Containment Systems
3-5. Fraction Collection
3-6. On-Line Injector
3-7. Analysis
Chapter 4: Animal Preparation and Surgery
4-1. Working with Living Animals: Ethical Considerations
4-2. Anesthesia
4-3. Implanting Linear and Loop Microdialysis Probes
4-4. Stereotaxic Surgery for Brain Microdialysis
4-5. Microdialysis of the Vascular System
4-6. Shunt Approach to Monitoring Bile
Chapter 5: The Microdialysis Experiment
5-1. Microdialysis of the Anesthetized Rat
5-2. Microdialysis of the Awake Rat
5-3. Using the Awake Animal Systems
5-4. Inserting a Brain Probe in an Awake Animal
5-5. Sample Handling
5-6. Histology
Chapter 6: Monitoring Pharmacokinetics and Drug Metabolism Using Microdialysis
6-1. Applications for Microdialysis in Pharmacokinetics
6-2. An In Vitro Metabolic Study
6-3. Quantitative Microdialysis
6-4. Monitoring Pharmacokinetics of Acetaminophen in Subcutaneous Tissue
6-5. Monitoring Acetaminophen Metabolism On-Line with a Liquid Chromatograph
6-6. Data Interpretation and Determination of Pharmacokinetic Parameters
Chapter 7: In Vivo Ultrafiltration
7-1. Introduction
7-2. Physiological Basis of In Vivo Ultrafiltration
7-3. Practical Considerations
Chapter 8: Setting Up the Ultrafiltration System
8-1. System Set-Up
8-2. UF Probe Preparation and Implantation
8-3. Laboratory Experiments
Chapter 9: References
9-1. Perfusion Fluids
9-2. In Vitro Recovery and Estimation Techniques
9-3. Anesthesia
9-4. Stereotaxic Surgery
9-5. Ultrafiltration
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