BASi LCEC Flowcells

BASi has developed a
variety of flow cells to fit a variety of applications. How do you make a choice? To begin, the analyte in most cases determines
the working electrode material. For most oxidizable/reducible organic compounds, a glassy carbon electrode is used, for
acetylcholine a platinum or wired glassy carbon is used(since enzymatic derivatization yields H2O2 for detection), Hg/Au is used to determine thiols/disulfides, a copper electrode for carbohydrates, gold for pulsed
electrochemical techniques-call BASi and we will discuss detection of your analyte.
Second, is the column of
standard size or is it microbore? In many instances a cell used for standard chromatography can be optimized for a microbore system
by the simple change of gasket thickness-thus reducing the cell-dead-volume. What flow pattern, cross-flow or radial-flow, will
give optimal response, thus resulting in lower detection limits? Generally radial-flow cells are best suited for microbore systems
and cross-flow for standard systems. If fraction collection or connection to a second detector (UV, fluorescence, or MS) is
required, then the cross-flow with reference port configuration (MF-1092) should be used.
The following sections describe a number of options. Call BASi if it is not clear to you which cell best fits your
system/application. A complete flowcell consists of two separate BASi part numbers:
- The Auxiliary Electrode includes a phenolic base, arms, and exit tube.
- The Cell Kit includes a glassy carbon working electrode, gasket, reference electrodes, the retaining hardware for
these electrodes, and a polishing kit.
In many cases several types of Cell Kits are compatible with a particular Auxiliary Electrode.
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