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Microdialysis Training

BASi offers on-site training for a variety of surgical and experimental procedures, including microdialysis. To request a quote for training, contact us. For those who do not have the time or resources for on-site training, we offer the Laboratory Microdialysis Sampling Training Manual.

The operation and format of the Laboratory Training Manual of Microdialysis and In Vivo UF Sampling on CD-ROM is similar to a web browser, and includes it s own specialized viewer. If printed, it would contain approximately 200 pages of text and graphics. There are approximately 150 photographs and illustrations, more than 50 of which are full-color, full-screen photographs illustrating probe implantations. Contact BASi for more information. Learn microdialysis in the comfort of your own lab.

Laboratory Training Manual of Microdialysis and In Vivo UF Sampling

Chapter 1: Overview of In Vivo Bioanalytical Sampling Techniques

  • 1-1. The Ideal Tool
  • 1-2. Open Cannula Techniques
  • 1-3. Microdialysis
  • 1-4. Ultrafiltration

Chapter 2: Preparation for Microdialysis

  • 2-1. Selecting a Probe
  • 2-2. Instrumentation Requirements
  • 2-3. Perfusion Fluids
  • 2-4. Preparing and Plumbing Microdialysis Probes
  • 2-5. In Vitro Recovery
  • 2-6. An In Vitro Recovery Experiment

Chapter 3: Setting Up a Microdialysis System: An Overview

  • 3-1. Start-Up
  • 3-2. Surgery
  • 3-3. Perfusion System
  • 3-4. Animal Containment Systems
  • 3-5. Fraction Collection
  • 3-6. On-Line Injector
  • 3-7. Analysis

Chapter 4: Animal Preparation and Surgery

  • 4-1. Working with Living Animals: Ethical Considerations
  • 4-2. Anesthesia
  • 4-3. Implanting Linear and Loop Microdialysis Probes
  • 4-4. Stereotaxic Surgery for Brain Microdialysis
  • 4-5. Microdialysis of the Vascular System
  • 4-6. Shunt Approach to Monitoring Bile

Chapter 5: The Microdialysis Experiment

  • 5-1. Microdialysis of the Anesthetized Rat
  • 5-2. Microdialysis of the Awake Rat
  • 5-3. Using the Awake Animal Systems
  • 5-4. Inserting a Brain Probe in an Awake Animal
  • 5-5. Sample Handling
  • 5-6. Histology

Chapter 6: Monitoring Pharmacokinetics and Drug Metabolism Using Microdialysis

  • 6-1. Applications for Microdialysis in Pharmacokinetics
  • 6-2. An In Vitro Metabolic Study
  • 6-3. Quantitative Microdialysis
  • 6-4. Monitoring Pharmacokinetics of Acetaminophen in Subcutaneous Tissue
  • 6-5. Monitoring Acetaminophen Metabolism On-Line with a Liquid Chromatograph
  • 6-6. Data Interpretation and Determination of Pharmacokinetic Parameters

Chapter 7: In Vivo Ultrafiltration

  • 7-1. Introduction
  • 7-2. Physiological Basis of In Vivo Ultrafiltration
  • 7-3. Practical Considerations

Chapter 8: Setting Up the Ultrafiltration System

  • 8-1. System Set-Up
  • 8-2. UF Probe Preparation and Implantation
  • 8-3. Laboratory Experiments

Chapter 9: References

  • 9-1. Perfusion Fluids
  • 9-2. In Vitro Recovery and Estimation Techniques
  • 9-3. Anesthesia
  • 9-4. Stereotaxic Surgery
  • 9-5. Ultrafiltration